Objectives

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Refine and share the present understanding of natural tissue environment with specific regard to 3-dimensional (3-D) organization and gradient of chemical and mechanical signals.

Replace the inefficient, unphysiological 2-D in vitro models with “3-D total guidance” models that more closely mimic the in vivo milieu.

Reduce the number of animals used to address similar questions with a particular emphasis on the concept of non-invasive investigations.

Develop and harmonize more physiological models and select the best culture system in order to mimic better what happens in vivo.

Determine and standardize the most adequate in vitro system according to the specific pathology and the scientific question/pharmacological management.

Create a database listing and implementing all existing models to avoid duplication of research in different European member states.

Capacity-building Objectives

The objectives described above are specifically designed to obtain consistent and measurable results that will allow to achieve:

  • Improvements of resources and technologies for generating refined in vitro models that more closely mimic the in vivo environment.
  • Standardized and validated new 3-D total guidance in vitro systems and ready to use culture kits.
  • A database of existing models, to facilitate the choice of the most adequate in vitro system according to the specific pathology and the scientific question.
  • An up-to-date bibliographic compilation of the relevant literature, an expert opinion to define needs and a dissemination platform for the stakeholders.

The data obtained will be relevant for the development of tools to communicate about translational research to health care professionals, to representatives from industry and to the public. They will lead to long-term relationships and collaborations between the partners of this Action to develop disease-specific networks implementing 3-D total guidance in vitro models.